Phylogenomics for Chagas Disease Vectors of the Rhodnius Genus (Hemiptera, Triatominae): What We Learn From Mito-Nuclear Conflicts and Recommendations

We provide in this study a very large DNA dataset on Rhodnius species including 36 samples representing 16 valid of the three groups, pictipes, prolixus and pallescens. Samples were sequenced at low-depth with whole-genome shotgun sequencing (Illumina technology). Using phylogenomics 15 mitochondrial genes (13.3 kb), partial nuclear rDNA (5.2 kb) 51 protein-coding (36.3 we resolve sticking points phylogeny. At level, confirmed species-specific status R. montenegrensis marabaensis agree synonymy taquarussuensis neglectus. also invite to revisit milesi that is more likely nasutus . proposed define robustus complex comprises four close relative species: marabaensis, montenegrensis, robustus. As Psammolestes tertius was included clade, strongly recommend reclassifying as group molecular data consistently supports clustering pictipes pallescens related each other than they are group. Moreover, comparing tree topologies, our results demonstrated various introgression events occurred all laboratory strains but wild specimens. introgressions frequently group, involving pairwise neglectus A genome wide analysis highlighted an event between stali brethesi suggested gene flow colombiensis, ecuadoriensis The sylvatic distribution Brazil (Pará state) monophyly detected extensive selective pressure genes, performing separate phylogenies take advantages mito-nuclear conflicts order have comprehensive evolutionary vision genus.